How does Gln-tRNA synthetase aminoacylate the correct tRNA with the correct amino acid?
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چکیده
منابع مشابه
Human histidyl-tRNA synthetase: recognition of amino acid signature regions in class 2a aminoacyl-tRNA synthetases.
We have determined the sequence of cDNA for the human histidyl-tRNA synthetase (HRS) in a hepatoma cell line and confirmed it in fetal myoblast and fibroblast cell lines. The newly determined sequence differs in 48 places, including insertions and deletions, from a previously published sequence. By sequence specific probing and by direct sequencing, we have established that only the newly deter...
متن کاملA tryptophanyl-tRNA synthetase/tRNA pair for unnatural amino acid mutagenesis in E. coli.
The genetic incorporation of unnatural amino acids (UAAs) with novel structures and properties into proteins in living cells has provided a powerful new tool to both probe and modify protein structure and function. The desired UAA is encoded by a nonsense or a frameshift codon using an engineered aminoacyl-tRNA synthetase (aaRS)/tRNA pair that is orthogonal to the host cell (i.e., not cross-rea...
متن کاملExpanding tRNA recognition of a tRNA synthetase by a single amino acid change.
Aspartyl-tRNA synthetase (AspRS) occurs in two types: the discriminating enzyme (D-AspRS) forms only Asp-tRNA(Asp), whereas the nondiscriminating enzyme (ND-AspRS) also synthesizes Asp-tRNA(Asn), which is a required intermediate for protein synthesis in many organisms. We attempted to expand the tRNA recognition of the discriminating Thermococcus kodakaraensis AspRS to that of a ND-AspRS by in ...
متن کاملAmino acid toxicities of Escherichia coli that are prevented by leucyl-tRNA synthetase amino acid editing.
Leucyl-tRNA synthetase (LeuRS) has evolved an editing function to clear misactivated amino acids. An Escherichia coli-based assay was established to identify amino acids that compromise the fidelity of LeuRS and translation. Multiple nonstandard as well as standard amino acids were toxic to the cell when LeuRS editing was inactivated.
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 1996
ISSN: 0108-7673
DOI: 10.1107/s0108767396092781